Controllable synthesis of uniform flower-shaped covalent organic framework microspheres as absorbent for solid-phase extraction of trace 2,4-dichlorophenol.

Controllable synthesis of micro-flower covalent organic frameworks (MFCOFs) with controllable size, monodisperse, spherical, and beautiful flower shape was realized by using 2,5-diformylfuran (DFF) and p-phenylenediamine (p-PDA) as building blocks at room temperature. High-quality MFCOFs (5 - 7 µm) were synthesized by controlling the kind of solvent, amounts of monomers, catalyst content, and reaction time. The synthesized MFCOFs possessed uniform mesopores deriving from the intrinsic pores of frameworks and wide-distributed pores belonging to the gap between the petals. The MFCOFs-packed solid-phase extraction (SPE) column shows adsorption capacity of about 8.85 mg g-1 for 2,4-dichlorophenol (2,4-DCP). The MFCOF-based SPE combined with the HPLC method was established for the determination of 2,4-DCP in environmental water. The linear range of this method is 20-1000 ng mL-1 (R2 > 0.9994), and limit of detection (S/N = 3) is 10.9 ng mL-1. Spiked recoveries were 94.3-98.5% with relative standard deviations lower than 2.3%.

Magnetic porous carbon material derived from imine-linked covalent organic frameworks for magnetic solid phase extraction of trace chlorine-containing herbicides in soil.

Here, imine-linked covalent organic frameworks coated Fe3O4 microspheres were fabricated and employed as the self-template to prepare magnetic porous carbon material. The magnetic solid phase extraction (MSPE) performance of such magnetic covalent organic frameworks derived porous carbons (CMCOFs) were studied for the first time, and the improved MSPE performance was verified. The variations of chemical and material properties in the carbonization processes were studied, and it was found that the CMCOFs carbonated at 400°C exhibited highest adsorption efficiencies for chlorine-containing herbicides due to the formation of nitrile components at this stage. The CMCOFs retained high adsorption efficiencies (above 90 %) to chlorine-containing herbicides at wide pH range (3-12) and high salt concentration. The CMCOFs-based MSPE coupled with HPLC technique was in good potential for analysis of trace chlorine-containing herbicides in soil samples. Under the optimized conditions, this approach displayed short extraction and elution time (5 and 8 min) and low limits of detection (0.35-5.5 ng/mL) for chlorine-containing herbicides. The recoveries of spiked analytes and the relative standard deviations in real soil samples were 81.86 %-110.9 % and less than 5.92 %, respectively. This study provides an efficient method for the analysis of trace chlorine-containing herbicides in complex samples, as well as give some inspiration on material modulation by controlled carbonization to achieve improved sorption performances.

Vanadium carbide MXene: as a reductant for the synthesis of gold nanoparticles and its biosensing application.

Vanadium carbide MXene (V2C) acts as a new type of two-dimensional (2D) graphene-like transition metal material that has attracted research interest. V2C has been widely used in various fields due to its excellent physical and chemical properties. Herein, the self-assembled V2C@gold nanoparticles (V2C@AuNPs) are prepared by water bath process at 80 °C. With the addition of glutathione (GSH), the absorbance (Abs.) at 550 nm of V2C@AuNPs was decreased. Therefore, an optical sensor is developed to detect GSH based on the properties of V2C@AuNPs. Under the optimal conditions, the detection range is 1-32 µM and the detection limit is 0.099 µM. Furthermore, the proposed GSH sensor exhibits high sensitivity, high selectivity, strong stability, and excellent recovery. The work will expand the application of V2C in biosensing.

Engineered Schwann Cell-Based Therapies for Injury Peripheral Nerve Reconstruction.

Compared with the central nervous system, the adult peripheral nervous system possesses a remarkable regenerative capacity, which is due to the strong plasticity of Schwann cells (SCs) in peripheral nerves. After peripheral nervous injury, SCs de-differentiate and transform into repair phenotypes, and play a critical role in axonal regeneration, myelin formation, and clearance of axonal and myelin debris. In view of the limited self-repair capability of SCs for long segment defects of peripheral nerve defects, it is of great clinical value to supplement SCs in necrotic areas through gene modification or stem cell transplantation or to construct tissue-engineered nerve combined with bioactive scaffolds to repair such tissue defects. Based on the developmental lineage of SCs and the gene regulation network after peripheral nerve injury (PNI), this review summarizes the possibility of using SCs constructed by the latest gene modification technology to repair PNI. The therapeutic effects of tissue-engineered nerve constructed by materials combined with Schwann cells resembles autologous transplantation, which is the gold standard for PNI repair. Therefore, this review generalizes the research progress of biomaterials combined with Schwann cells for PNI repair. Based on the difficulty of donor sources, this review also discusses the potential of "unlimited" provision of pluripotent stem cells capable of directing differentiation or transforming existing somatic cells into induced SCs. The summary of these concepts and therapeutic strategies makes it possible for SCs to be used more effectively in the repair of PNI.

Magnetic phenolic resin core-shell structure derived carbon microspheres for ultrafast magnetic solid-phase extraction of triazine herbicides.

In this study, monodisperse magnetic carbon microspheres were successfully synthesized through the carbonization of phenolic resin encapsulated Fe3 O4 core-shell structures. The magnetic carbon microspheres showed high performance in ultrafast extraction and separation of trace triazine herbicides from environmental water samples. Under optimized conditions, both the adsorption and desorption processes could be achieved in 2 min, and the maximum adsorption capacity for simazine and prometryn were 387.6 and 448.5 µg/g. Coupled with high-performance liquid chromatography-ultraviolet detection technology, the detection limit of triazine herbicides was in the range of 0.30-0.41 ng/mL. The mean recoveries ranged from 81.44 to 91.03% with relative standard deviations lower than 7.47%. The excellent magnetic solid-phase extraction performance indicates that magnetic carbon microspheres are promising candidate adsorbents for the fast analysis of environmental contaminants.

Serology-Based Model for Personalized Epithelial Ovarian Cancer Risk Evaluation.

This study aimed to establish a prognosis-prediction model based on serological indicators in patients with epithelial ovarian cancer (EOC). Patients initially diagnosed as ovarian cancer and surgically treated in Fudan University Shanghai Cancer Center from 2014 to 2018 were consecutively enrolled. Serological indicators preoperatively were collected. A risk model score (RMS) was constructed based on the levels of serological indicators determined by receiver operating characteristic curves. We correlated this RMS with EOC patients' overall survival (OS). Finally, 635 patients were identified. Pearson's χ2 results showed that RMS was significantly related to clinical parameters. Kaplan−Meier analysis demonstrated that an RMS less than 3 correlated with a longer OS (p < 0.0001). Specifically, significant differences were perceived in the survival curves of different subgroups. Multivariate Cox analysis revealed that age (p = 0.015), FIGO stage (p = 0.006), ascites (p = 0.015) and RMS (p = 0.005) were independent risk factors for OS. Moreover, RMS combined with age, FIGO and ascites could better evaluate for patients' prognosis in DCA analyses. Our novel RMS-guided classification preoperatively identified the prognostic subgroups of patients with EOC and showed higher accuracy than the conventional method, meaning that it could be a useful and economical tool for tailored monitoring and/or therapy.

Wettability tunable metal organic framework functionalized high internal phase emulsion porous monoliths for fast solid-phase extraction and sensitive analysis of hydrophilic heterocyclic amines.

Surface wettability greatly influences the adsorptive, catalytic, and diffuse performances of a porous material. To realize the improved adsorption performance to hydrophilic heterocyclic amines (HAs), polymeric high internal phase emulsions (polyHIPEs) that can be tuned from hydrophobic to hydrophilic is synthesized by facilely regulating the amount of metal organic frameworks (MOFs). The water contact angle of the MOFs and polyHIPEs hybrids (MOFs@polyHIPEs) decreases from 133° to 0° as the amount of amide-modified MOFs increases from 0% to 10%. The hydrophilization of divinybenzene (DVB) based polyHIPEs by MOFs hybridization significantly enhances their adsorption performance and enables them to be suitable for the solid phase extraction (SPE) of hydrophilic HAs. Under the optimized conditions, the MOFs@polyHIPEs achieve adsorption capacities ranging from 42.89 to 86.71 µg/g for HAs through the π-π interaction and hydrogen bonding. The adsorption follows the pseudo-second-order kinetic model, and the nitrogen atoms in/on the imidazole ring are identified as the active adsorption sites for hydrogen bonding. This SPE method, along with HPLC-MS detection, provides detection limits of HAs as low as 0.00020-0.00040 ng/mL. This work offers a feasible strategy in tuning the surface wettability of polyHIPEs without post-modification to achieve high-efficiency enrichment and analysis of HAs.

Aptamer functionalized and reduced graphene oxide hybridized porous polymers SPE coupled with LC-MS for adsorption and detection of human α-thrombin.

In this study, reduced graphene oxide (rGO) hybridized high internal phase emulsions were developed and polymerized as porous carriers for aptamer (5'/5AmMC6/-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3') modification to enrich human α-thrombin from serum. The structure and properties of the materials were confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscope (FT-IR), and X-ray photoelectron spectra (XPS). The adsorption ability and selectivity were studied and the thrombin was detected with liquid chromatography-mass spectrometry (LC-MS). The adsorption of thrombin onto the sorbent was achieved within 30 min and the desorption was realized using 5.0 mL of acetonitrile/water (80/20, v/v). The thrombin was quantified by LC-MS according to its characteristic peptide sequence of ELLESYIDGR.

The effects of hemocyanin on T cells cultured in vitro.

As a broad-spectrum antibiotic, gentamicin is used extensively in T cell culturing in vitro, but preliminary studies have identified that T cell activity is significantly affected by gentamicin. In the present study, the hemocyanin from Litopenaeus vannamei (L. vannamei) was selected as an additive for T cell cultures in vitro. Compared with those in the control group, the cell quantity exhibited no significant difference, and the formation rate of cell colony increased gradually with increases in the hemocyanin concentration. Additionally, flow cytometry assays identified that cluster of differentiation (CD)3+CD4+ and CD4+CD25+ T cells in the 0.2 µg/ml hemocyanin (Hem 3) group were all significantly increased. Furthermore, cell cycle analysis demonstrated that T cells in the G0/G1 phase were significantly decreased in the Hem 3 group compared with in the control, 0.05 µg/ml (Hem 1) and 0.1 µg/ml (Hem 2) groups, and cells in the S phase were significantly elevated in the Hem 3 group compared with in the control and Hem 1 groups. In addition, MTT analysis indicated that the cytotoxicity of T cells towards HepG2 cells was significantly increased in the Hem 3 group compared with in the control, Hem 1 and Hem 2 groups. Taken together, the present study identified that hemocyanin may improve the proliferation and cytotoxicity of T cells, and the results supported the use of hemocyanin in T cell adoptive immunotherapy.